The research is part of a project promoted by the Municipality of Florence in order to planning a program of HIV prevention and promoting the access to free HIV test. Theoretical approaches come from studies about health attitudes, healthy behavior and perceived control on wellbeing (Albarracin, Kumkale, &Johnson,2004; Davis, Hughes, Sloan, Tang, & McMaster, 2009; Fishbein,2008), different interventions in health promotion (Boone & Lefkowitz, 2004; Fortenberry, Tu, Harezlak, Katz & Orr, 2002; Harper, Bangi, Contreras, Pedraza Tolliver & Vess, 2004), importance of professionals (Durantini, Albarracin, Mitchell, Earl, & Gillette, 2006; Fisher, Bryan, & Misovich, 2002), particularly general practitioners (Schreibman & Friedland, 2003). The purpose of the research is to identify useful indications for community based interventions addressed to adult people. Our aim is collecting information about professionals’ perception of citizens’ attitude and behavior in order to find strengths and weakness in health promotion activities carried out by public services. The qualitative study was aimed to investigate attitudes towards the primary and secondary prevention, and willingness and motivation to taking HIV test. Participants were physicians, psychologists and volunteers, involved in health services addressed both to the whole of population (e.g. professionals working in surgeries and in consulting rooms) and to HIV positive people. Instrument was a semi-structured interview for exploring professionals’ perception of patients’ attitudes towards this disease, risky behaviors, preventive behaviors and evaluation about health services practices. Findings show a positive evaluation of available health services and their cooperation in facing the problem, despite difficulties in involving all the professionals. Prevention follows well-established paths but is not able to reach all citizens. There is a need of a wider information, and prevention involving people as individuals and groups. General practitioners may have a critical and important role, because of their trust relationship with their patients, and can be able to disseminate preventive practices.