Bombus impatiens (Hymenoptera: Apidae) display reduced pollen foraging behavior when marked with bee tags vs. paint
DOI:
https://doi.org/10.17161/jom.v0i62.5679Keywords:
bumble bee, cyanoacrylate, sonication, pollination, buzz pollination, Bombus impatiens, Apoidea, Apidae, Apinae, Bombini, AnthophilaAbstract
Numbered bee tags, developed for marking honey bees (Apis mellifera Linnaeus), are glued to the mesosoma of many bees to uniquely identify them. We recorded whether or not bees sonicated to collect pollen after being marked, and we compared the sonication frequency, sonication length, and wing beat frequency of Bombus (Pyrobombus) impatiens Cresson that were tagged with bee tags vs. marked with paint. We found that bees with tags glued to their mesosoma had no significant change in wing beat frequency, sonication frequency, or sonication length, relative to bees that were marked with paint; however, we found that the probability of collecting pollen via sonication after being marked was much lower for bees marked with bee tags vs. paint.Metrics
References
Audacity. 2015. Free, open source, cross-platform software for recording and editing sounds. Version 2.0.5. [http://audacity.sourceforge.net/; last accessed 8 November 2015]
Boiteau, G., F. Meloche, C. Vincent, & T.C. Leskey. 2009. Effectiveness of glues used for harmonic radar tag attachment and impact on survival and behavior of three insect pests. Environmental Entomology 38(1): 168–175.
Buchmann, S.L. 1983. Sonication pollination in angiosperms. In: Jones, C.E., & R.J. Little (Eds.), Handbook of Experimental Pollination Biology: 73–113. van Nostrand Reinhold Company, Inc.; New York, NY; xviii+558 pp.
Capaldi, E.A., A.D. Smith, J.L. Osborne, S.E. Fahrbach, S.M. Farris, D.R. Reynolds, A.S. Edwards, A. Martin, G.E. Robinson, G.M. Poppy, & J.R. Riley. 2000. Ontogeny of orientation flight in the honeybee revealed by harmonic radar. Nature 403(6769): 537–540.
Cheng, R. 2013. qtlmt: Tools for Mapping Multiple Complex Traits. R package version 0.1-3. [http://CRAN.R-project.org/package=qtlmt; last accessed 8 November 2015]
Crall, J.D., N. Gravish, A.M. Mountcastle, & S.A. Combes. 2015. BEEtag: A low-cost, image-based tracking system for the study of animal behavior and locomotion. PLoS ONE 10(9): e0136487.
De Luca, P.A., & M. Vallejo-Marín. 2013. What’s the ‘buzz’ about? The ecology and evolutionary significance of buzz-pollination. Current Opinion in Plant Biology 16(4): 429–435.
De Souza, A.R., B. Ribeiro, J. Newton, & F. Prezoto. 2012. Paint marking social wasps: An evaluation of behavioral effects and toxicity. Entomologia Experimentalis et Applicata 144(2): 244–247.
Eltz, T., W.M. Whitten, D.W. Roubik, & K.E. Linsenmair. 1999. Fragrance collection, storage, and accumulation by individual male orchid bees. Journal of Chemical Ecology 25(1): 157–176.
Fox, J., & S. Weisberg. 2011. An R Companion to Applied Regression [2nd Edition]. Sage; Thousand Oaks, CA; 472 pp.
Hagbery, J., & J.C. Nieh. 2012. Individual lifetime pollen and nectar foraging preferences in bumble bees. Naturwissenschaften 99(10): 821–832.
Hagen, M., M. Wikelski, & W.D. Kissling. 2011. Space use of bumblebees (Bombus spp.) revealed by radio-tracking. PLoS ONE 6(5): e19997.
Horikoshi, M., & Y. Tang. 2015. ggfortify: Data Visualization Tools for Statistical Analysis Results. R package version 0.0.4. [http://CRAN.R-project.org/package=ggfortify; last accessed 8 November 2015]
Hsu, J., 1996. Multiple Comparisons: Theory and Methods. CRC Press; Boca Raton, FL; ix+296 pp.
Hurlbert, S.H. 1984. Pseudoreplication and the design of ecological field experiments. Ecological Monographs 54(2): 187–211.
King, M.J. 1993. Sonication foraging mechanism of bumble bees. Journal of Apicultural Research 32(1): 41–49.
King, M.J., S.L. Buchmann, & H. Spangler. 1996. Activity of asynchronous flight muscle from two bee families during sonication (sonicationing). Journal of Experimental Biology 199(10): 2317–2321.
Ligges, U., S. Krey, O. Mersmann, & S. Schnackenberg. 2013. tuneR: Analysis of Music. R package version 1.2.1. [http://r-forge.r-project.org/projects/tuner/; last accessed 8 November 2015].
Manso, R., M. Fortin, R. Calama, & M. Pardos. 2013. Modeling seed germination in forest tree species through survival analysis. The Pinus pinea L. case study. Forest Ecology and Management 289: 515–524.
Medeiros, J., & A. Araújo. 2014. Workers’ extra-nest behavioral changes during colony fission in Dinoponera quadriceps (Santschi). Neotropical Entomology 43(2): 115–121.
Milinski, M. 1997. How to avoid seven deadly sins in the study. Advances in the Study of Behavior 26: 159–180.
Osborne, J.L., & I.H. Williams. 2001. Site constancy of bumble bees in an experimentally patchy habitat. Agriculture, Ecosystems and Environment 83(1): 129–141.
Osborne, J.L., S.J. Clark, R.J. Morris, I.H. Williams, J.R. Riley, A.D. Smith, D.R. Reynolds, & A.S. Edwards. 1999. A landscape?scale study of bumble bee foraging range and constancy, using harmonic radar. Journal of Applied Ecology 36(4): 519–533.
Packer, L. 2005. The influence of marking upon bee behaviour in circle tube experiments with a methodological comparison among studies. Insectes Sociaux 52(2): 139–146.
Poissonnier, L.A., A.L. Jackson, & C.J. Tanner. 2015. Cold and CO2 narcosis have long-lasting and dissimilar effects on Bombus terrestris. Insectes Sociaux 62(3): 291–298.
R Core Team. 2015. R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing; Vienna, Austria. [https://www.R-project.org/; last accessed 8 November 2015]
Reynolds, A.M., J.L. Swain, A.D. Smith, A.P. Martin, & J.L. Osborne. 2009. Honeybees use a Lévy flight search strategy and odour-mediated anemotaxis to relocate food sources. Behavioral Ecology and Sociobiology 64(1): 115–123.
Scheiner, R., C.I. Abramson, R. Brodschneider, K. Crailsheim, W.M. Farina, S. Fuchs, B. Grünewald, S. Hahshold, M. Karrer, G. Koeniger, N. Koeniger, R. Menzel, S. Mujagic, G. Radspieler, T. Schmickl, C. Schneider, A.J. Seigel, M. Szopek, & R. Thenius. 2013. Standard methods for behavioural studies of Apis mellifera. Journal of Apicultural Research 52(4): 1–58.
Sueur, J., T. Aubin, & C. Simonis. 2008. Seewave: A free modular tool for sound analysis and synthesis. Bioacoustics 18(2): 213–226.
Switzer, C.M., K. Hogendoorn, S. Ravi, & S.A. Combes. 2016. Shakers and head bangers: Differences in sonication behavior between Australian Amegilla murrayensis (blue-banded bees) and North American Bombus impatiens (bumblebees). Arthropod-Plant Interactions 10(1): 1–8.
Tenczar, P., C.C. Lutz, V.D. Rao, N. Goldenfeld, & G.E. Robinson. 2014. Automated monitoring reveals extreme interindividual variation and plasticity in honeybee foraging activity levels. Animal Behaviour 95: 41–48.
Therneau, T.M., & P.M. Grambsch. 2000. Modeling Survival Data: Extending the Cox Model. Springer; New York, NY; xiii+350 pp.
Unwin, D.M., & S.A. Corbet. 1984. Wingbeat frequency, temperature and body size in bees and flies. Physiological Entomology 9(1): 115–121.
Walker, T.J., & S.A. Wineriter. 1981. Marking techniques for recognizing individual insects. Florida Entomologist 64(1): 18–29.
Wickham, H. 2009. ggplot2: Elegant Graphics for Data Analysis. Springer; New York, NY; vii+213 pp.
Wilson, E.E., D. Holway, & J.C. Nieh. 2006. Cold anaesthesia decreases foraging recruitment in the New World bumblebee, Bombus occidentalis. Journal of Apicultural Research 45(4): 169–172.
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