Lipid Peroxidation as a Biomarker for Heavy Metal Stress in Plants
DOI:
https://doi.org/10.17161/mjusc.v4i1.23998Palabras clave:
Lipid peroxidation, Malondialdehyde (MDA), Heavy metal stress, Copper sulfate (CuSO₄), Oxidative stress, TBARS assay, Plant biomarkers, Root elongation assay, Environmental toxicity, PhytoremediationResumen
Heavy metal contamination in soil poses a significant threat to plant health, agriculture, and ecological stability. This study investigates the potential of lipid peroxidation, specifically malondialdehyde (MDA) accumulation, as a biomarker for oxidative stress in plants exposed to heavy metals. Plants were subjected to varying concentrations of Copper Sulfate, and physiological parameters such as root length were recorded to assess growth inhibition. To quantify lipid peroxidation, a thiobarbituric acid reactive substances (TBARS) assay was performed, with a standard curve generated using 1,1,3,3-tetramethoxypropane (TMP) as an MDA equivalent. Results revealed a dose-dependent increase in MDA levels correlating with reduced root growth, indicating enhanced oxidative damage under metal stress. These findings support the use of lipid peroxidation as a reliable indicator of heavy metal toxicity and lay the groundwork for its application in phytoremediation studies and environmental monitoring.
Referencias
1. Halliwell, B. & Gutteridge, J. M. Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem. J. 219, 1–14 (1984).
2. Janero, D. R. Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue
injury. Free Radic. Biol. Med. 9, 515–540 (1990).
3. Dhindsa, R., Plumb-Dhindsa, P. & Thorpe, T. Leaf senescence and lipid peroxidation. J Exp Bot 32, 93–101 (1981).
4. Baryla, A., Laborde, C., Montillet, J. L., Triantaphylidès, C. & Chagvardieff, P. Evaluation of lipid peroxidation as a toxicity bioassay
for plants exposed to copper. Environ. Pollut. Barking Essex 1987 109, 131–135 (2000).
5. D, S. & M, M. Standardizing the Methods for Breaking Seed Dormancy to Enhance Germination of Gloriosa superba Seeds. Expert
Opin. Environ. Biol. 04, (2016).
6. Hajiboland, R. An evaluation of the efficiency of cultural plants to remove heavy metals from growing medium. Plant Soil Environ. 51,
(2005).
7. Heath, R. L. & Packer, L. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 125, 189–198 (1968).
8. Bird, R. P. & Draper, H. H. Comparative studies on different methods of malonaldehyde determination. Methods Enzymol. 105, 299–305 (1984).
9. Rooney, C. P., Zhao, F.-J. & McGrath, S. P. Soil factors controlling the expression of copper toxicity to plants in a wide range of European soils. Environ. Toxicol. Chem. 25, 726–732 (2006).
10. Copper-induced stress in Solanum nigrum L. and antioxidant defense system responses - Fidalgo - 2013 - Food and Energy Security - Wiley Online Library. https://onlinelibrary.wiley.com/doi/full/10.1002/fes3.20?utm_source=chatgpt.com.
Descargas
Publicado
Número
Sección
Licencia
Derechos de autor 2025 Soham Kawade, Jack Treml, Sonia Thomas, Melissa Daggett, Brendan Mattingly
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial 4.0.
© The Author(s)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International license.
