Effects of Interleukin-17 on Expression of CD40 and CD40L in T and B Cell Lines

Abstract

Interleukin-17 (IL-17, also known as IL-17A) plays a role in immune regulation and pathogenesis of various diseases. Cluster of differentiation 40 (CD40) and its ligand CD40L are critical for T and B cell communications and activation of immune responses. This study investigated the effects of IL-17A on CD40L expression in Jurkat T cells and CD40 expression in Mino B cells. The cells were treated with IL-17A (40 ng/mL for Jurkat cells and 20 ng/mL for Mino cells) for durations ranging from 0 to 24 hours. Protein expression levels were assessed using Western blotting combined with densitometric analysis. IL-17A treatment resulted in an increase in CD40L expression in Jurkat cells, although this change did not reach statistical significance (P > 0.05). In contrast, CD40 expression in Mino cells was significantly decreased at 24 h compared to the control group (*P < 0.05). These findings indicate that IL-17A differentially regulates the expression of co-stimulatory molecules in T and B cells, potentially contributing to its modulatory effects on T–B cell interactions.